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QuPath is an open, powerful, flexible, extensible software platform for whole slide image analysis.

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QuPath v0.2.3 is available here.

This is a minor release focused on fixing bugs; see the changelog for details.

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June 2020: QuPath v0.2.0 now available!

The first Edinburgh release of QuPath is available here.

More than three years since v0.1.2 and a lot has changed.
Highlights include:

  • Entirely new pixel classifier (link)
  • Rewritten object classifiers (link)
  • New methods of thresholding images (link)
  • New & improved tools to create & adjust annotations (link)
  • Much more support for multiplexed images (link)
  • Updated object hierarchy (link)
  • Bigger, better, smarter projects (link)
  • Export images & annotations, including pyramidal OME-TIFFs (link)
  • Many bug fixes, performance improvements… and a lot more (link)

Find the full documentation at https://qupath.readthedocs.io

Important! It is not recommended to mix different version of QuPath for analysis. If you started a project in v0.1.2, it is probably best to continue with that version - or start again with v0.2.0.

Other news

April 2020: QuPath webinar at NEUBIAS Academy

The QuPath webinar at NEUBIAS Academy is now on YouTube.

April 2020: From Samples to Knowledge workshop online

Videos from the recent QuPath workshop held at the La Jolla Institute for Immunology are now on YouTube

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Please remember to cite the QuPath paper if you use it in your work!

Bankhead, P. et al. QuPath: Open source software for digital pathology image analysis. Scientific Reports (2017). https://doi.org/10.1038/s41598-017-17204-5

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QuPath is developed at the University of Edinburgh.

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The software was originally created at the Centre for Cancer Research & Cell Biology at Queen’s University Belfast, as part of research projects funded by Invest Northern Ireland and Cancer Research UK.